Journal: eLife

Article Title: Glycan-based shaping of the microbiota during primate evolution

doi: 10.7554/eLife.67450

Figure Lengend Snippet: ( A ) Schematic showing infection of Ggta1 -/- mice with a cecal inoculum from either Ggta1 -/- mice or Rag2 -/- Ggta1 -/- mice. ( B ) Survival of Ggta1 -/- (n = 14) mice after infection with a cecal inoculum from Ggta1 -/- mice and of Ggta1 -/- (n = 13) mice after infection with a cecal inoculum from Rag2 -/- Ggta1 -/- mice; two experiments. ( C ) Survival of Igh-J +/+ Ggta1 -/- (n = 9) and Igh-J -/- Ggta1 -/- (n = 8) mice after infection with a cecal inoculum from Ggta1 -/- mice; two experiments. ( D ) Survival of Tcrb +/+ Ggta1 -/- (n = 8) and Tcrb -/- Ggta1 -/- (n = 12) mice infected as in ( C ); two experiments. ( E ) Survival of Rag2 +/+ Ggta1 -/- (n = 9) and Rag2 -/- Ggta1 -/- (n = 23) mice infected as in ( C ); five experiments. ( F ) Representative plots showing depletion of CD11b + Ly6G + cells in the blood 24 hr after Anti-Gr1 Ab injection (i.p.) in the same mice as in . ( G ) Representative plots showing depletion of CD11b + F4/80 + and CD11b + Ly6C + cells in the peritoneal lavage 72 hr after injection (i.p.) with Clodronate liposomes. ( H ) Survival of Ggta1 -/- mice receiving PBS liposomes (n = 7) or Clodronate liposomes (n = 8), 72 hr before infection (i.p.) with cecal inoculum from Ggta1 -/- mice; two experiments. p Values in ( B, C, D, E, H ) calculated with log-rank test. Peritoneal cavity (PC). ns: not significant.

Article Snippet: Antibody , Anti-Mouse Monoclonal Gr1 (Clone: RB6-8C5) , Bio-Rad , N/A , 300 μg in 200 μL PBS per mouse.

Techniques: Infection, Injection, Liposomes

Journal: eLife

Article Title: Glycan-based shaping of the microbiota during primate evolution

doi: 10.7554/eLife.67450

Figure Lengend Snippet: ( A ) Schematic showing infection of Rag2 -/- Ggta1 -/- mice with a cecal inoculum from either Ggta1 -/- or Rag2 -/- Ggta1 -/- mice. ( B ) Survival of Rag2 -/- Ggta1 -/- (n = 9) mice after infection with a cecal inoculum from Ggta1 -/- mice, and from Rag2 -/- Ggta1 -/- (n = 9) mice after infection with a cecal inoculum from Rag2 -/- Ggta1 -/- mice; two experiments. ( C ) Colony-forming units (CFU) of aerobic (Ae) and anaerobic (An) bacteria in Rag2 -/- Ggta1 -/- mice (n = 5 per group), 24 hr after infection as in ( B ); two experiments. ( D ) Schematic showing infection of Rag2 -/- Ggta1 +/+ mice with a cecal inoculum from either Ggta1 +/+ or Rag2 -/- Ggta1 +/+ mice. ( E ) Survival of Rag2 -/- Ggta1 +/+ (n = 5) mice after infection with a cecal inoculum from Ggta1 +/+ mice, and Rag2 -/- Ggta1 +/+ (n = 5) mice after infection with a cecal inoculum from Rag2 -/- Ggta1 +/+ mice; one experiment. ( F ) CFU of Ae and An bacteria in Rag2 -/- Ggta1 -/- mice (n = 4 per group), 24 hr after infection as in ( E ); one experiment. ( G ) Survival of Ggta1 -/- mice receiving vehicle (PBS) (n = 9) or Anti-Gr1 Ab (n = 8), 24 hr before infection with cecal inoculum from Ggta1 -/- mice; two experiments. ( H ) CFU of Ae and An bacteria in Ggta1 -/- mice receiving vehicle (PBS) (n = 4–5) or Anti-Gr1 Ab (n = 4–5), 24 hr after infection as in ( G ); five experiments. Symbols ( C, F, H ) represent individual mice. Red lines ( C, F, H ) correspond to median values. p Values in ( B, E, G ) calculated with log-rank test and in ( C, F, H ) with Mann-Whitney test. Peritoneal cavity (PC). *p<0.05, **p<0.01, ***p<0.001, ns: not significant.

Article Snippet: Antibody , Anti-Mouse Monoclonal Gr1 (Clone: RB6-8C5) , Bio-Rad , N/A , 300 μg in 200 μL PBS per mouse.

Techniques: Infection, Bacteria, MANN-WHITNEY

Journal: eLife

Article Title: Glycan-based shaping of the microbiota during primate evolution

doi: 10.7554/eLife.67450

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-Mouse Monoclonal Gr1 (Clone: RB6-8C5) , Bio-Rad , N/A , 300 μg in 200 μL PBS per mouse.

Techniques: Probiotics, Expressing, Bacteria, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Serial Dilution, Liposomes, Control, In Vivo, Staining, Software

Journal: Oncotarget

Article Title: Oncogenic KIT-induced aggressive systemic mastocytosis requires SHP2/PTPN11 phosphatase for disease progression in mice

doi:

Figure Lengend Snippet: A. Representative images of spleens isolated from DBA/2 mice at day 13 following retro-orbital injection of saline (sham), or P815-NT cells, or P815-KD cells (5 × 10 3 in 100 μl; 3-4 mice/group; 3 experiments). B. Graph depicts spleen weights from saline-injected sham mice (n=3), or mice injected with P815-NT or P815–KD cells (n=11) at day 13 (mean ± SD; * indicates significant difference between groups, p <0.05). C. Splenocytes isolated from mice described above were analyzed by flow cytometry. Representative histograms are shown for splenocytes stained with FITC-Gr1/PE-CD11b/PEcy5-B220, with mean values shown for single and double positive populations (n=3; results are representative of 3 independent experiments). D. Graph depicts absolute cell numbers (x10 6 ) for Gr1 + , CD11b + , and B220 + cells in spleen from mice described above (* indicates significant difference between NT and KD, p <0.05). E. Representative histograms are shown for splenocytes stained with PE-KIT/PEcy5-CD45, with mastocytoma cells identified as double positives, from mice described above. F. Graph depicts the absolute mastocytoma cell numbers in spleen (mean ± SD; n=10; * indicates significant difference between NT and KD, p <0.05).

Article Snippet: [ ] Antibodies for flow cytometry included: PE-conjugated anti-mouse CD117 (2B8; BD Bioscience), PE/cy5-conjugated anti-mouse CD45 (eBioscience), PE conjugated anti-mouse CD11b (M1/70; Biolegend), PE/cy5-conjugated anti-mouse CD45R (B220) (RA3-6B2; Biolegend), FITC anti-mouse Gr1 (RB6-8C5; Cedarlane).

Techniques: Isolation, Injection, Saline, Flow Cytometry, Staining

Journal: Oncotarget

Article Title: Oncogenic KIT-induced aggressive systemic mastocytosis requires SHP2/PTPN11 phosphatase for disease progression in mice

doi:

Figure Lengend Snippet: A. BM cells were isolated from DBA/2 mice at day 13 following retro-orbital injection of saline (sham), or P815-NT cells, or P815-KD cells. BM cells were stained with FITC-Gr1/PE-CD11b/PEcy5-B220 and analyzed by flow cytometry. Graph depicts absolute cell numbers (x10 6 ) for Gr1 + , CD11b + , and B220 + cells in BM from sham (n=3), or mice injected with P815-NT or P815-KD cells (mean ± SD, n=11; * indicates significant difference between NT and KD, p <0.05). B. Representative histograms are shown for BM cells stained with PE-KIT/PEcy5-CD45, with mastocytoma cells identified as double positives, from mice described above. C. Graph depicts the absolute mastocytoma cell numbers in BM (mean ± SD; n=11; * indicates significant difference between NT and KD, p <0.05).

Article Snippet: [ ] Antibodies for flow cytometry included: PE-conjugated anti-mouse CD117 (2B8; BD Bioscience), PE/cy5-conjugated anti-mouse CD45 (eBioscience), PE conjugated anti-mouse CD11b (M1/70; Biolegend), PE/cy5-conjugated anti-mouse CD45R (B220) (RA3-6B2; Biolegend), FITC anti-mouse Gr1 (RB6-8C5; Cedarlane).

Techniques: Isolation, Injection, Saline, Staining, Flow Cytometry

Journal: Oncotarget

Article Title: Oncogenic KIT-induced aggressive systemic mastocytosis requires SHP2/PTPN11 phosphatase for disease progression in mice

doi:

Figure Lengend Snippet: A. Single cell suspensions from peripheral blood were isolated from DBA/2 mice at day 13 following retro-orbital injection of saline (sham), or P815-NT cells, or P815-KD cells. Following lysis of erythrocytes, cells were stained with FITC-Gr1/PE-CD11b/PEcy5-B220, and analyzed by flow cytometry. Graph depicts absolute cell numbers (x10 6 ) for Gr1 + , CD11b + , and B220 + cells in peripheral blood from sham (n=3), or mice injected with P815-NT or P815-KD cells (mean ± SD, n=9 from 3 separate experiments). B. Representative histograms are shown for peripheral blood cells stained with PE-KIT/PEcy5-CD45, with mastocytoma cells identified as double positives, from mice analyzed in 3 independent experiments as described above. C. Graph depicts the absolute mastocytoma cell numbers in peripheral blood (mean ± SD; n=9 from 3 separate experiments; * indicates significant difference between NT and KD, p <0.05).

Article Snippet: [ ] Antibodies for flow cytometry included: PE-conjugated anti-mouse CD117 (2B8; BD Bioscience), PE/cy5-conjugated anti-mouse CD45 (eBioscience), PE conjugated anti-mouse CD11b (M1/70; Biolegend), PE/cy5-conjugated anti-mouse CD45R (B220) (RA3-6B2; Biolegend), FITC anti-mouse Gr1 (RB6-8C5; Cedarlane).

Techniques: Isolation, Injection, Saline, Lysis, Staining, Flow Cytometry